AN OPTIMISED PIPELINE FOR PARALLEL IMAGE-BASED QUANTIFICATION OF GENE EXPRESSION AND GENOTYPING AFTER IN SITU HYBRIDISATION

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines.A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH).Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-type and mutant siblings can be done blinded and in pa

Primary clear cell sarcoma of soft tissue (CCSST) arising from Grommets the pleura is an infrequent occurrence, with only two cases reported in the English literature.We report a rare case of pleural CCSST with unusual clinical features and unconventional morphology.A 68-year-old man presented with a large right pleural effusion that developed foll